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Figure 4 | Genome Biology

Figure 4

From: Tiling microarray analysis of rice chromosome 10 to identify the transcriptome and relate its expression to chromosomal architecture

Figure 4

Analysis of intergenic TARs of japonica chromosome 10. (a) The 988 japonica chromosome 10 intergenic TARs distributed by length. (b) RNA gel blotting analysis of selected japonica intergenic TARs. Probes for the intergenic TARs shown in this panel were derived from corresponding PCR-amplified TAR sequences from japonica rice genomic DNA. (c) Probes shown in this panel were derived from RT-PCR amplification of the corresponding TARs from poly(A)+ RNA. (d) The rice cDNAs for eIF4A and actin2 were used as loading controls. 5 μg of RNA from the four sources - root, shoot, panicle, and suspension cell culture - that were used for probing tiling microarrays were used for RNA blot analysis here.

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