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Figure 1 | Genome Biology

Figure 1

From: Tiling microarray analysis of rice chromosome 10 to identify the transcriptome and relate its expression to chromosomal architecture

Figure 1

Processing the rice chromosome 10 tiling microarray hybridization data. (a) Distribution of fluorescence intensity of all positive and negative features of the four indica N Arrays. (b) All eight distributions were scaled to have a uniform intensity peak value at 8 (log2). (c) Mathematic model for determination of signal probes. A bimodal distribution of log2 background-adjusted intensity of all positive features is used to model the noise as a normal distribution by mirroring the distribution of low intensity (< 6 of log2). A cutoff value corresponding to a 90% confidence level to reject noise probes according to the modeled noise distribution is indicated. (d) Distribution of hybridization rate in the exonic and intronic regions of rice chromosome 10. Hybridization rate (HR) is calculated as the ratio of the number of signal probes against the total number of interrogating probes per kilobase of sequence.

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