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Table 2 Expression of cysteine, serine and aspartic proteases in POPULUSDB and in a microarray analysis

From: A genomic approach to investigate developmental cell death in woody tissues of Populustrees

Protease

Cluster

Transcript

Most similar Arabidopsis gene

E

Number of ESTs in each library

Expression ratio B/A

     

I

X

Y

G

Tot

      

Cysteine

11539

Cysteine endopeptidase CysEP

At5g50260

7.1e-146

0

4

0

0

30

*14.9

     
 

1250

Papain-like cysteine proteinase RD19A

At4g39090

1.4e-133

51

23

2

3

211

1.2

*3.0

1.1

*3.4

*4.1

1.4

 

289

Papain-like cysteine proteinase

At5g43060

1.3e-103

16

1

1

1

32

0.9

1.4

1.4

   
 

3027

Vacuolar processing enzyme

At4g32940

9.2e-200

4

8

1

1

34

*3.0

*3.8

*3.1

*3.3

  
 

3310

Papain-like cysteine endopeptidase XCP2

At1g20850

1.7e-144

0

5

1

2

14

*0.7

*0.2

    
 

4102

Papain-like cysteine proteinase SAG12

At5g45890

8.8e-111

13

0

0

0

14

1.0

0.9

    
 

5498

Papain-like cysteine proteinase AALP

At5g60360

3.4e-129

4

1

0

0

18

1.3

*1.8

*2.3

   
 

7212

Papain-like cysteine proteinase RD21A

At1g47128

2.6e-169

28

5

2

3

59

2.2

     

Serine

10138

Subtilisin-like serine protease

At1g01900

9.1e-107

0

3

0

0

5

*5.0

     
 

4702

Prolyl endopeptidase

At1g76140

1.6e-52

4

0

0

0

9

1.1

1.2

    
 

4905

Serine carboxypeptidase III-like protein

At3g10410

9.8e-194

0

4

0

2

16

1.6

1.3

    
 

4995

Subtilisin-like serine protease

At1g20160

7.0e-68

0

5

0

0

6

*12.2

     
 

8771

DegP protease

At3g27925

6.2e-60

9

0

0

0

9

1.6

     

Aspartic

3648

Aspartic-type endopeptidase (phytepsin)

At1g11910

3.5e-208

6

3

0

1

26

0.9

*2.5

    
 

4286

Aspartic-type endopeptidase (family A1)

At5g43100

3.5e-41

3

1

0

0

4

1.0

1.2

1.2

   
 

541

Aspartic-type endopeptidase (family A1)

At2g03200

3.0e-124

0

6

0

10

19

1.1

0.7

    
 

915

Aspartic-type endopeptidase (phytepsin)

At1g11910

1.3e-105

3

3

1

1

35

1.2

1.0

1.3

   
  1. Distribution of the ESTs is shown in the cDNA libraries of senescing leaves (I), fiber death (X), virus/fungal infected leaves (Y) and tension wood (G) of the POPULUSDB. Tot, the total number of ESTs in the POPULUSDB. Note that the Y library was partially subtracted and only 1,395 ESTs were sequenced from this library. E represents BLASTX statistics for the most similar Arabidopsis gene. The expression ratio was calculated between gene expression in sample B (fiber-cell death) and sample A (early fiber development). Several transcripts were represented on the microarray by multiple cDNA clones. The asterisks denote statistically significant differences in gene expression when P < 0.001 and B > 0. The complete microarray data, including statistics, can be found in Additional data file 2.