FLP mapping in Drosophila. (a) Crossing scheme used to map mutations generated in the FRT background and recombined with an EP line. The different classes of recombinants recovered in the F2 generation are shown. (b) Big head phenotypes of the hippo null allele hpo42-20 (1) and the VI.29 mutation (2). A wild-type control is shown in (3). (c) FLP mapping of the VI.29 mutation on chromosome 2R. Analysis of the different classes of recombinants places the mutation between markers 2R096 and 2R109 (dashed red line). Informative recombinants are boxed in red. ND, not determined or no data as a result of PCR reaction failure.