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Table 9 Quantitative real-time PCR results for selected chromosome 21 genes in Table 1

From: Primary and secondary transcriptional effects in the developing human Down syndrome brain and heart

Gene name

Chromosome

p value

Cerebrum

Cerebellum

Astrocyte

Heart

   

Microarray

qPCR

Microarray

qPCR

Microarray

qPCR

Microarray

qPCR

Pituitary tumor-transforming 1 interacting protein (PTTG1IP)

21

1.50E-07

1.52

3.85 ± 0.38

1.42

1.46 ± 0.62

1.65

2.42 ± 1.16

1.74

1.41 ± 0.16

ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (ATP5O)

21

5.11E-07

1.69

1.52 ± 0.20

1.75

0.91 ± 0.16

1.34

1.48 ± 0.72

1.69

3.52 ± 1.22

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J)

21

2.47E-06

1.84

1.25 ± 0.14

1.40

1.19 ± 0.12

1.51

1.73 ± 0.78

2.23

5.19 ± 1.60

Down syndrome critical region gene 3 (DSCR3)

21

1.44E-05

1.82

2.76 ± 0.44

1.86

0.79 ± 0.38

3.40

2.50 ± 0.62

0.98

2.06 ± 0.06

Chromosome 21 segment HS21C048, zinc finger protein 294 (ZNF294)

21

3.39E-05

1.71

2.58 ± 0.24

1.42

1.30 ± 0.42

1.63

1.60 ± 0.24

1.66

1.51 ± 0.14

Superoxide dismutase 1 (SOD1)

21

5.62E-05

2.12

1.72 ± 0.24

1.57

1.44 ± 0.12

1.55

2.75 ± 1.37

1.74

2.86 ± 1.52

Cystatin B (stefin B) (CSTB)

21

7.75E-05

1.69

1.21 ± 0.13

1.49

1.21 ± 0.14

1.18

1.92 ± 0.56

1.30

1.88 ± 0.07

Phosphofructokinase, liver (PFKL)

21

1.93E-04

1.16

2.17 ± 0.14

1.92

1.35 ± 0.08

1.61

2.07 ± 0.44

1.11

2.23 ± 0.55

Pyridoxal (pyridoxine, vitamin B6) kinase (PDXK)

21

2.82E-04

2.73

2.99 ± 0.40

2.13

1.79 ± 0.20

2.21

2.18 ± 1.01

1.44

1.75 ± 0.09

Collagen, type VI, alpha 1 (COL6A1)

21

5.04E-04

1.72

3.16 ± 0.24

1.39

0.75 ± 0.06

1.45

2.57 ± 1.28

1.39

1.97 ± 0.76

Ubiquitin specific protease 16 (USP16)

21

5.33E-04

1.80

2.75 ± 0.48

1.30

1.33 ± 0.36

1.67

1.70 ± 0.50

1.67

3.40 ± 1.54

SMT3 suppressor of mif two 3 homolog 1 (yeast) (SMT3H1)

21

6.27E-04

1.68

1.66 ± 0.42

1.50

0.93 ± 0.08

1.56

2.56 ± 1.26

1.40

4.07 ± 2.71

Mitochondrial ribosomal protein L39 (MRPL39)

21

7.48E-04

1.44

1.25 ± 0.08

1.04

1.40 ± 0.27

1.24

2.41 ± 1.14

1.41

1.91 ± 0.29

Interferon gamma receptor 2 (IFNGR2)

21

8.16E-04

1.36

2.21 ± 0.29

1.36

1.78 ± 0.12

1.48

1.82 ± 0.44

1.27

1.27 ± 0.41

Human homolog of ES1 (zebrafish) protein (C21orf33)

21

1.02E-03

1.48

5.44 ± 2.14

1.71

1.85 ± 0.40

1.06

1.21 ± 0.24

1.99

1.38 ± 0.16

Chaperonin containing TCP1, subunit 8 (theta) (CCT8)

21

1.45E-03

1.38

2.51 ± 0.44

1.29

3.64 ± 0.92

1.94

1.09 ± 0.07

1.77

0.75 ± 0.04

Tryptophan rich basic protein (WRB)

21

2.18E-03

1.89

5.21 ± 2.08

1.28

1.94 ± 0.39

1.83

2.87 ± 2.23

1.94

1.78 ± 0.44

HMT1 hnRNP methyl-transferase-like 1 (HRMT1L1)

21

3.62E-03

1.37

1.50 ± 0.54

1.44

1.93 ± 0.36

0.96

1.46 ± 0.50

1.45

1.65 ± 0.05

  1. Data were normalized to the HPRT housekeeping gene. P values were derived from ANOVA of microarray data. Values for microarray data are fold-regulation. For each quantitative PCR (qPCR) experiment, values were determined by measuring samples in duplicate at multiple concentrations (mean ± standard error). Each experiment was performed independently at least three times.
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Genome Biology

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