Global expression changes resulting from loss of telomeric DNA in fission yeast

Table 1 Genes with significantly altered expression in trt1^- cells

Category	Examples	GC	Circ
Acetyltransferase (2)	ppr1⁺, SPBC1271.07c*	2-0	1-0
Alcohol metabolism (2)	SPCC24B10.20, SPAPB24D3.08c	2-0	0-0
Amino acid and derivative metabolism (6)	SPBC119.03, SPBPB21E7.04c, SPAC139.05*	5-1	3-1
Carbohydrate metabolism (14)	eno102⁺, tms1⁺, fbp1⁺, SPCC663.08c*	13-1	8-0
Cell organization (3)	eng1⁺, SPBC8E4.10c, SPAC11D3.01c	2-1	0-0
Cofactor metabolism (2)	SPAC513.07, SPAC2E1P3.04 ^§	1-1	0-0
DNA maintenance and recombination (3)	SPAC212.11, trt1*⁺	2-1	0-1
Energy production (5)	SPBC23G7.10c, SPAC513.02, SPBC1773.06c*	5-0	2-0
Ion homeostasis (2)	zym1⁺, SPBC947.05c*	2-0	1-0
Meiosis and sporulation^‡(5)	mfm2^+†, meu3RC⁺^†, meu8⁺^†, meu27⁺, SPBC354.08c*^†	4-1	0-0
Methyltransferase (1)	SPAC1B3.06c*	1-0	0-0
Mitochondrial energy and proteins (10)	cox1⁺, cox3⁺, cob⁺, atp6⁺, atp8⁺, atp9⁺	10-0	0-0
Nucleotide metabolism (2)	SPBC1683.06c, SPCC965.14c	1-0	1-0
Proteolysis (6)	isp6⁺, SPBC1685.05, SPCC338.12	6-0	1-0
Pseudogene (7)	SPBC16E9.16c, SPBPB21E7.08	7-0	3-0
RNA binding and regulation (3)	SPCC74.09, SPAC4G8.03c	3-0	1-0
Non-coding RNA (1)	meu3RC	1-0	0-0
Signal transduction (2)	hri1⁺, SPBC725.06c*	2-0	1-0
Stress response (8)	hsp16⁺, cta1⁺, hsp9⁺, ish1⁺, pyp2⁺	8-0	3-0
Sulfur metabolism (2)	gst2⁺, SPBC1198.01*	1-0	2-0
Transcription (3)	aes1⁺, SPAC30.02c, SPBC1105.14	3-0	1-0
Transporter (6)	cta3⁺, SPCC1840.12*	5-1	2-0
Unknown function/hypothetical protein (29)	SPAC25H1.01c*	29-0	24-0

The total number of genes in each category is indicated in parenthesis. For each category, the number listed before the hyphen is the number of genes with at least two instances of upregulated expression, and the number after the hyphen is the number of genes with at least two cases of downregulated expression. GC, growth curve; Circ, strains C1 and C5, where numbers represent changes that occurred in both strains. *Putative function. ^†Meiosis-associated genes with changed expression in the CESR [16]. ^‡This category contains genes that may also appear in other categories. All other categories are nonredundant. ^§SPAC2E1P3.04 appears to have been deleted from the genome in all strains except WT 3, WT 5 and C1.

ISSN: 1474-760X