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Table 1 Distribution of the toxic inserts between the different genetic objects

From: Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

Genetic objects represented

Number of toxic inserts

Percentage of total

Mean size ± SD (nucleotides) (minimum-maximum)

Phenotypes

Inserts encoding artificial peptides

    

3/0, 3/1

3/2

2/0, 2/1

1/0

 

In-frame ORF fragments

493

68.7

743 ± 311 (220-2,120)

375

87

23

8

_

Antiparallel ORF fragments

68

9.6

532 ± 247 (140-1,220)

37

11

12

8

53

Out-of-frame ORF fragments

53

7.5

733 ± 306 (170-1,620)

12

11

22

8

12

Intergenic regions

41

6.0

625 ± 358 (170-1,820)

13

4

16

8

27

LTRs

2

0.3

595 (320-1,120)

1

0

0

1

1

Ty elements

15 (10)

2.1

633 ± 265 (320-870)

7

4

2

2

_

Y' elements

9 (3)

1.2

678 ± 370 (320-1,320)

9

0

0

0

6

RNA genes

4

0.5

662 ± 246 (470-1,020)

3

0

1

0

3

2 μm plasmid

17 (10)

2.4

564 ± 288 (170-1,220)

13

3

1

0

5

Mitochondrial DNA

12

1.7

483 ± 201 (200-920)

9

3

0

0

10

Total

714

100

703 ± 313 (140-2,120)

479

123

77

35

117

  1. The first column indicates nature of sequence in toxic inserts. Second and third columns contain, respectively, actual number of inserts of each type and corresponding percentages. For Tys, Y' and 2 μm plasmid, numbers in brackets represent numbers of in-frame fragments of natural ORFs. The fourth column shows the mean size of insert in nucleotides ± standard deviation (SD) with minimum and maximum sizes in brackets. Scoring of each type of phenotype is shown in the next four columns. The last column shows the number of inserts in which artificial ORFs of more than 24 codons were detected.