Statistical modeling for selecting housekeeper genes

Table 5 Primers for housekeeper genes

	Length (bases)	GC(%)	*Tm (°C)
PSMC4 (UniGene reference Hs.211594 - Gene ID: 5704)
GGCATGGACATCCAGAAG	18	55.6	60
CCACGACCCGGATGAAT	17	58.8	61
Amplified fragment	190	61	90
MRPL19 (UniGene reference Hs.44024 - Gene ID: 9801)
GGGATTTGCATTCAGAGATCAG	22	45	62
GGAAGGGCATCTCGTAAG	18	56	61
Amplified fragment	182	44	83
PUM1 (UniGene reference Hs.153834 - Gene ID:9698)
TGAGGTGTGCACCATGAAC	19	53	61
CAGAATGTGCTTGCCATAGG	20	50	61
Amplified fragment	187	53	87
SF3A1 (UniGene reference Hs.406277 -Gene ID:10291)
GGAGGATTCTGCACCTTCTAA	21	47	61
GCGGTAGTAGGCATGGTAA	19	52	60
Amplified fragment	196	48	85
ACTB (UniGene reference Hs.426930 - Gene ID:60)
TTCCTGGGCATGGAGTC	17	59	60
CAGGTCTTTGCGGATGTC	18	55	60
Amplified fragment	84	55	84
^†GAPD (UniGene reference Hs.169476 - Gene ID:2597)
AACAGCCTCAAGATCATCAGC	21	48	63
GGATGATGTTCTGGAGAGCC	20	55	62
Amplified fragment	198	56	89

*Primers Tm determined using Tm Utility with algorithms adapted from Santa Lucia [23]. The Tm for the amplified fragment is the empirical Tm. ^†Primers for GAPD [20].

ISSN: 1474-760X