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Table 1 Cost of materials required to make one inkjet oligonucleotide array

From: POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Item Purchase Unit Price ($) Required Unit Line cost ($)
Glass slides 140 each 20.85 1 each 0.15
Nano-Strip glass cleaner 3,800 ml 37.18 3.2 ml 0.03
Sodium hydroxide 1,000 g 43.15 1.25 g 0.05
Hydrochloric acid 2,500 ml 42.96 0.42 ml 0.01
Methanol 4,000 ml 18.53 6.4 ml 0.03
Epoxysilane 93 ml 21.90 0.06 ml 0.01
Rain-X silane solution 200 ml 4.00 11 ml 0.22
Isopropanol 4,000 ml 29.09 4 ml 0.03
Acetone 4,000 ml 30.69 4 ml 0.03
Slide derivatization subtotal       0.56
Nitrogen 4,000 scf 90.00 225 scf 5.06
Acetonitrile 1,000 ml 38.08 250 ml 9.52
0.02 M Iodine THF/Pyr/H2O 450 ml 36.00 32 ml 2.56
2.5% DCA in DCM 450 ml 18.00 32 ml 1.28
dA-CE phosphoramidite 250 mg 3.50 14 mg 0.20
Ac-dC-CE phosphoramidite 250 mg 3.50 14 mg 0.20
dG-CE phosphoramidite 250 mg 3.50 14 mg 0.20
dT-CE phosphoramidite 250 mg 3.50 14 mg 0.20
Tetrazole 1,000 mg 35.00 56 mg 1.96
Ammonia 2,500 ml 37.67 8 ml 0.12
Methylamine 1,000 ml 23.05 8 ml 0.18
Ethanol 3,800 ml 41.70 11 ml 0.12
3-methoxypropionitrile 1,000 ml 41.90 1.5 ml 0.06
2-methyl glutaronitrile 1,000 ml 39.15 1.5 ml 0.06
Chemical synthesis subtotal       21.71
Lifterslip coverslips 40 each 38.65 1 each 0.97
DIG Easy-Hyb solution 600 ml 228.00 0.1 ml 0.04
Control oligo (Bodipy) 12,000 pmol 250.00 10 pmol 0.21
Wash buffers -   - -   0.05
Test hybridization subtotal       1.26
Septa 72 each 48.18 0.05 each 0.03
Drierite 2,000 g 82.53 14 g 0.58
Syringe, 1 ml 100 each 13.62 0.3 each 0.04
Syringe, 5 ml 100 each 12.74 0.3 each 0.04
26G needles 100 each 8.37 0.3 each 0.03
Molecular sieves 500 g 31.12 0.7 g 0.04
Inkjet head 1 each 85.00 0.003 each 0.26
Exhaust filter cartridge 1 each 79.00 0.0015 each 0.12
Parafilm 250 ft 28.22 0.05 ft 0.01
Miscellaneous materials subtotal       1.14
Total       24.68
  1. Labor, safety equipment, and waste-disposal expenses are not included. Arrays printed on commercial epoxysilane slides require an additional $7 to $12.