Table 1 Cost of materials required to make one inkjet oligonucleotide array
From: POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer
Item | Purchase | Unit | Price ($) | Required | Unit | Line cost ($) |
|---|---|---|---|---|---|---|
Glass slides | 140 | each | 20.85 | 1 | each | 0.15 |
Nano-Strip glass cleaner | 3,800 | ml | 37.18 | 3.2 | ml | 0.03 |
Sodium hydroxide | 1,000 | g | 43.15 | 1.25 | g | 0.05 |
Hydrochloric acid | 2,500 | ml | 42.96 | 0.42 | ml | 0.01 |
Methanol | 4,000 | ml | 18.53 | 6.4 | ml | 0.03 |
Epoxysilane | 93 | ml | 21.90 | 0.06 | ml | 0.01 |
Rain-X silane solution | 200 | ml | 4.00 | 11 | ml | 0.22 |
Isopropanol | 4,000 | ml | 29.09 | 4 | ml | 0.03 |
Acetone | 4,000 | ml | 30.69 | 4 | ml | 0.03 |
Slide derivatization subtotal | Â | Â | Â | Â | Â | 0.56 |
Nitrogen | 4,000 | scf | 90.00 | 225 | scf | 5.06 |
Acetonitrile | 1,000 | ml | 38.08 | 250 | ml | 9.52 |
0.02 M Iodine THF/Pyr/H2O | 450 | ml | 36.00 | 32 | ml | 2.56 |
2.5% DCA in DCM | 450 | ml | 18.00 | 32 | ml | 1.28 |
dA-CE phosphoramidite | 250 | mg | 3.50 | 14 | mg | 0.20 |
Ac-dC-CE phosphoramidite | 250 | mg | 3.50 | 14 | mg | 0.20 |
dG-CE phosphoramidite | 250 | mg | 3.50 | 14 | mg | 0.20 |
dT-CE phosphoramidite | 250 | mg | 3.50 | 14 | mg | 0.20 |
Tetrazole | 1,000 | mg | 35.00 | 56 | mg | 1.96 |
Ammonia | 2,500 | ml | 37.67 | 8 | ml | 0.12 |
Methylamine | 1,000 | ml | 23.05 | 8 | ml | 0.18 |
Ethanol | 3,800 | ml | 41.70 | 11 | ml | 0.12 |
3-methoxypropionitrile | 1,000 | ml | 41.90 | 1.5 | ml | 0.06 |
2-methyl glutaronitrile | 1,000 | ml | 39.15 | 1.5 | ml | 0.06 |
Chemical synthesis subtotal | Â | Â | Â | Â | Â | 21.71 |
Lifterslip coverslips | 40 | each | 38.65 | 1 | each | 0.97 |
DIG Easy-Hyb solution | 600 | ml | 228.00 | 0.1 | ml | 0.04 |
Control oligo (Bodipy) | 12,000 | pmol | 250.00 | 10 | pmol | 0.21 |
Wash buffers | - | Â | - | - | Â | 0.05 |
Test hybridization subtotal | Â | Â | Â | Â | Â | 1.26 |
Septa | 72 | each | 48.18 | 0.05 | each | 0.03 |
Drierite | 2,000 | g | 82.53 | 14 | g | 0.58 |
Syringe, 1 ml | 100 | each | 13.62 | 0.3 | each | 0.04 |
Syringe, 5 ml | 100 | each | 12.74 | 0.3 | each | 0.04 |
26G needles | 100 | each | 8.37 | 0.3 | each | 0.03 |
Molecular sieves | 500 | g | 31.12 | 0.7 | g | 0.04 |
Inkjet head | 1 | each | 85.00 | 0.003 | each | 0.26 |
Exhaust filter cartridge | 1 | each | 79.00 | 0.0015 | each | 0.12 |
Parafilm | 250 | ft | 28.22 | 0.05 | ft | 0.01 |
Miscellaneous materials subtotal | Â | Â | Â | Â | Â | 1.14 |
Total | Â | Â | Â | Â | Â | 24.68 |