Figure 4

Modularity of gene interactions. The 298 HU-selected strains were perturbed with other drugs, and GI values were analyzed by hierarchical clustering. The color intensity represents the magnitude of the GI, green being negative (synergistic effect of gene deletion), but note that the range of color intensity may be different for each perturbation (see Figure 2j and Additional data file 3) because the phenotypic noise, determined for replicates of the reference strain, is measured uniquely for each perturbation (see Figure 2i). Gene clusters were given numbers (on right) for ease of referral, based subjectively on their appearance with respect to the dendrogram branches (see also Additional data file 10). GI values are reported in Additional data file 9. The first two columns (C) indicate the GI for unperturbed deletion strains (synthetic complete media, no drug). '_gen' indicates data from the original genomic screen. Otherwise, data are from a single retest of selected strains. The other columns indicate drugs used for perturbation as follows (numbers following the abbreviation indicate the concentration): miconaz, miconazole (nM); TBHP, t-butyl hydroperoxide (mM); cyclohex, cycloheximide (ng/ml); HU, hydroxyurea (mM); cisplat, cisplatin (μM). The drug perturbations and the growth phenotypes for the reference strain under each perturbation are given in Additional data file 1. Gene names are from SGD and descriptions can be found in Additional data file 10 [47].