- Open Access
Cross-species comparison of genome-wide expression patterns
© BioMed Central Ltd 2004
- Published: 21 June 2004
The rapid accumulation of microarray data from multiple species provides unprecedented opportunities to study the evolution of biological systems. Recent studies have used cross-species comparisons of expression profiles to annotate gene functions, to draw evolutionary inferences concerning specific biological processes and to study the global properties of expression networks.
- Ortholog Pair
- Specific Biological Process
- Similar Biological Process
- Biological Interaction Network
- Global Network Property
The power of comparative genomic analysis relies on the assumption that important biological properties are often conserved across species. Cross-species sequence comparison has been widely used to infer gene function; but it is becoming apparent that sequence similarity is not always proportional to functional similarity [1, 2]. In fact, Gene Ontology (GO) terms  distinguish between molecular and biological functions, and although the amino-acid sequence may imply that a gene possesses a particular molecular function, spatiotemporal expression data is required to infer biological function - which cellular or biological process the gene product participates in. To determine the function of a gene precisely, therefore, we need to investigate not only its sequence characteristics but also its expression characteristics. An increasing number of genetic studies indicate that the divergent functions of many duplicate genes are reflected in the divergence of expression patterns rather than in differences between their coding sequences [4, 5]. On the other hand, changes in gene expression may often be associated with changes in function . The expression pattern of a gene can thus serve as a sensitive indicator of its function. An early study in this regard was performed by Su et al.  who measured the correlation between the expression profiles of human and mouse ortholog pairs across 16 tissues (the dataset has now been extended to over 50 tissues and is available online as the Novartis Gene Expression Atlas ). This work identified several cases in which the ortholog pairs have dissimilar expression patterns, and the authors were able to infer, for example, that human and mouse collagen XV have different physiological functions.
Functional analysis of microarray data often begins with the determination of which genes are significantly co-expressed. Apparent co-expression of genes will often be 'real', but it can also occur by chance as a result of the noisiness of microarray data, the complexity of transcriptional programs or simply as a function of the enormous number of comparisons that are being made. Gene pairs exhibiting co-expression in multiple species and across a large number of arrays in each species are most likely to be functionally relevant. This is because co-regulation of a pair of genes over large evolutionary distances implies that divergence in their expression profiles is mechanistically and/or adaptively constrained, and because a high correlation of expression caused by chance or noisiness in the data in one species is unlikely to occur in another species. The evolutionary conservation of co-expression patterns thus provides functional information that is orthogonal and complementary to that provided by sequence data.
Bergmann et al.  used a slightly different procedure to combine sequence and expression analysis (Figure 1b), focusing on six species. They started with a set of co-expressed genes, S a , known to be associated with a particular function in organism a, and identified the set of their sequence homologs, S b , in organism b using BLAST. Only a subset S b ' of S b was found to be co-expressed, and these genes were considered to be the functionally conserved homologs of S a . S b ' was further expanded to S b '' by including genes in organism b that are co-expressed with genes in S b ' but that do not share sequence similarity with genes in S a . As an example, the authors started with a set of heat-shock genes in yeast, successfully identified a set of co-regulated heat-shock genes in Escherichia coli and Caenorhabditis elegans, and showed that half a dozen more co-regulated genes in these latter species also have functions in the heat-shock response even though their orthologs are not annotated in this way in yeast. The results demonstrate that the extent of co-regulation increases drastically from S b to S b ' to S b ', leading to the conclusion that sequence-based functional annotation can be improved through the integration of expression data.
In addition to providing information about the function of individual genes, cross-species expression comparison can be used to analyze entire sets of genes to understand how system properties are conserved over evolution. Much has been written about the power-law connectivity of biological interaction networks: in protein interaction networks, rather than interactions occurring at random, it seems that certain key proteins have many more interactions with other proteins . This pattern is thought to arise as a result of the way in which interaction networks grow by the addition of new elements to existing networks. It now appears that power-law connectivity is also observed in the correlations between pairs of genes, both in metagene co-expression networks  and in the expression networks of different organisms . Certain features of gene-expression networks are likely to differ from those of protein networks, including the very high level of modularity seen in expression networks. There is also some suggestion that genes with high connectivity in a network are less dispensable to the organism and more likely to be evolutionarily conserved .
Many system properties have been shown to be different between species. Interestingly, Bergmann et al.  observed that most of the relations between functional modules differ between organisms. For example, heat-shock and protein-biosynthesis modules exhibit a strong negative correlation in the yeast and Drosophila expression data, but have a significant positive correlation in E. coli, C. elegans, Arabidopsis thaliana and human. In addition, genes involved in protein biosynthesis show tight co-regulation across a variety of conditions in yeast, but exhibit less significant co-expression in other organisms. This suggests that the transcriptional regulation of genes involved in protein biosynthesis plays a major role in the transcriptional program of unicellular organisms but a less dominant role in multicellular organisms. In some cases, the restriction of modules to one or two organisms reflects the modularity of tissue structure, for example in animal-specific signaling pathways and neuronal functions .
Besides the global expression modules, comparison of the expression patterns of genes involved in particular biological processes has the potential to provide more detailed and specific evolutionary information. This principle was first pursued by Alter et al. , who compared time points during the cell cycle between yeast and human using generalized singular value decomposition. This computational framework dissects expression patterns into those common to both species, as well as those that are exclusive to one dataset or the other. Another study by Rifkin et al.  investigated genome-wide expression variation between Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition - the start of metamorphosis. Extensive evolution of developmental gene expression was observed among these closely related species. Interestingly, both within the transcriptional network that controls metamorphosis and across the whole genome, the expression levels of transcription-factor genes appear to be more conserved than those of their downstream targets.
A more recent study compares genomic expression profiles during the aging process in D. melanogaster and C. elegans . The comparison is based on shared patterns of regulation for orthologous genes. Specifically, McCarroll et al.  calculated the Pearson's correlation of the log-transformed relative expression change of orthologous genes between middle-aged adults and young adults in both species. Correlations ranging from 0.14 to 0.18 were shown to be statistically significant by permutation procedures despite the very different tissue structure and absolute ages of the two organisms. Furthermore, grouping of genes by GO categories led the authors to observe a conserved pattern of regulation that most notably includes several genes of oxidative metabolism. Nevertheless, most transcriptional changes were specific to worms or to flies; for example, the repression of genes encoding collagens and the induction of genes encoding histones, transposases and DNA and/or RNA helicases are specific to the aging process in worms, whereas the activation of expression of cytochrome P450s, glycosylases and peptidoglycan receptors are specific to aging in Drosophila. In an intriguing sideline, more detailed comparisons revealed that both the conserved global pattern of change in gene expression and the conserved repression of oxidative metabolism genes were abruptly implemented in early adulthood in both organisms. These results suggest that changes in gene expression observed in adults are not solely implemented in response to cumulative damage, as hypothesized in one common model of the aging process . Instead, the timing of these conserved features of aging suggests that they are regulated by developmentally timed transcriptional regulation in young adults.
A critical assessment of the literature on the topic of cross-species comparisons of gene expression would probably start with the observation that some of the inferences are based on optimistic evaluation of very weak correlations. For example, where the correlations observed across species are reported [10, 15], they are less than 0.2, so most of the variation is not explained by shared expression across species. It is not a question of whether the cup is half full or half empty: clearly there is just a small mouthful left to swallow, but the evolutionary elixir is an appealing one. Tasty enough, it seems, to justify the annotation of gene function and the inference of regulatory conservation. The statistical justification for this is that with thousands of data points, observation of even small correlations is highly unlikely, as indicated by Monte Carlo simulations resulting in p values less than 10-10 .
A key potential difficulty in studies that use cross-species comparisons of gene expression is whether the available data for each organism provide a sufficient summary of the covariance structure of gene expression to facilitate reproducible comparison with other species. Current microarray data repositories are unbalanced in terms of the species represented: for example, data from human and yeast are much more abundant than those from fly and E. coli, and experimental conditions for each species also vary. To check the data sufficiency, Stuart et al.  randomly divided their compendium of datasets into two halves and evaluated the correspondence between expression networks. Just over 40% of the interactions they observed were significant in both halves, indicating that although these approaches are definitely sensitive to the number, and presumably nature, of conditions tested, there is still a strong enough signal to detect at least a portion of true interactions. McCarroll et al.  hint that there is likely to be considerable information to be found in detailed comparisons of specific biological processes, as they find some evidence for conservation of programs regulating larval and embryonic development in worm and fly, and of similar biological processes between more divergent organisms, such as sporulation in yeast and germline formation in C. elegans. Furthermore, there are certainly more sophisticated statistical approaches than simple evaluation of correlation between orthologous gene pairs that remain to be evaluated. These would include the incorporation of phylogenetic information and the use of Bayesian or mixture models to evaluate the significance of expression profiles in two or more species jointly.
Several studies using microarray expression data have suggested that there has been rapid divergence of expression between duplicated genes in human  and in yeast [6, 18]. Although paralogs may diverge in expression more rapidly than do single-copy genes , data from studies comparing the expression levels of duplicated genes within a species [6, 17, 18] will provide some context for interpreting cross-species comparisons of gene-expression profiles [9, 10]. More extensive datasets that evaluate gene expression in matched conditions (for example, similar genetic or environmental perturbations at the same developmental or life-history stages) are likely to improve the power of comparative studies. The signatures of conservation will probably remain subtle, but they will provide plenty of suggestions for hypothesis testing. A more detailed understanding of the conservation of regulatory systems will eventually also require careful attention to the mechanisms and patterns of transcriptional divergence, which after all lie at the heart of morphological, physiological and behavioral evolution.
- Gerlt JA, Babbitt PC: Can sequence determine function?. Genome Biol. 2000, 1: reviews0005.1-0005.10. 10.1186/gb-2000-1-5-reviews0005.View ArticleGoogle Scholar
- Wilson CA, Kreychman J, Gerstein M: Assessing annotation transfer for genomics: quantifying the relations between protein sequence, structure and function through traditional and probabilistic scores. J Mol Biol. 2000, 297: 233-249. 10.1006/jmbi.2000.3550.PubMedView ArticleGoogle Scholar
- Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al: Gene ontology: tool for the unification of biology. Nat Genet. 2000, 25: 25-29. 10.1038/75556.PubMedPubMed CentralView ArticleGoogle Scholar
- Lin XL, Lin YZ, Tang J: Relationships of human immunodeficiency virus protease with eukaryotic aspartic proteases. Methods Enzymol. 1994, 241: 195-224. 10.1016/0076-6879(94)41066-6.PubMedView ArticleGoogle Scholar
- Hanks M, Wurst W, Anson-Cartwright L, Auerbach AB, Joyner AL: Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2. Science. 1995, 269: 679-682.PubMedView ArticleGoogle Scholar
- Wagner A: Decoupled evolution of coding region and mRNA expression patterns after gene duplication: implications for the neutralist-selectionist debate. Proc Natl Acad Sci USA. 2000, 97: 6579-6584. 10.1073/pnas.110147097.PubMedPubMed CentralView ArticleGoogle Scholar
- Su AI, Cooke MP, Ching KA, Hakak Y, Walker JR, Wiltshire T, Orth AP, Vega RG, Sapinoso LM, Moqrich A, et al: Large-scale analysis of the human and mouse transcriptomes. Proc Natl Acad Sci USA. 2002, 99: 4465-4470. 10.1073/pnas.012025199.PubMedPubMed CentralView ArticleGoogle Scholar
- GNF SymAtlas. [http://symatlas.gnf.org/SymAtlas/]
- Stuart JM, Segal E, Koller D, Kim SK: A gene-coexpression network for global discovery of conserved genetic modules. Science. 2003, 302: 249-255. 10.1126/science.1087447.PubMedView ArticleGoogle Scholar
- Bergmann S, Ihmels J, Barkai N: Similarities and differences in genome-wide expression data of six organisms. PLoS Biol. 2004, 2: E9-10.1371/journal.pbio.0020009.PubMedPubMed CentralView ArticleGoogle Scholar
- Quackenbush J: Microarrays - guilt by association. Science. 2003, 302: 240-241. 10.1126/science.1090887.PubMedView ArticleGoogle Scholar
- Barabasi AL, Oltvai ZN: Network biology: understanding the cell's functional organization. Nat Rev Genet. 2004, 5: 101-113. 10.1038/nrg1272.PubMedView ArticleGoogle Scholar
- Alter O, Brown PO, Botstein D: Generalized singular value decomposition for comparative analysis of genome-scale expression data sets of two different organisms. Proc Natl Acad Sci USA. 2003, 100: 3351-3356. 10.1073/pnas.0530258100.PubMedPubMed CentralView ArticleGoogle Scholar
- Rifkin SA, Kim J, White KP: Evolution of gene expression in the Drosophila melanogaster subgroup. Nat Genet. 2003, 33: 138-144. 10.1038/ng1086.PubMedView ArticleGoogle Scholar
- McCarroll SA, Murphy CT, Zou S, Pletcher SD, Chin CS, Jan YN, Kenyon C, Bargmann CI, Li H: Comparing genomic expression patterns across species identifies shared transcriptional profile in aging. Nat Genet. 2004, 36: 197-204. 10.1038/ng1291.PubMedView ArticleGoogle Scholar
- Tower J: Aging mechanisms in fruit flies. Bioessays. 1996, 18: 799-807.PubMedView ArticleGoogle Scholar
- Makova KD, Li WH: Divergence in the spatial pattern of gene expression between human duplicate genes. Genome Res. 2003, 13: 1638-1645. 10.1101/gr.1133803.PubMedPubMed CentralView ArticleGoogle Scholar
- Gu Z, Nicolae D, Lu HH, Li WH: Rapid divergence in expression between duplicate genes inferred from microarray data. Trends Genet. 2002, 18: 609-613. 10.1016/S0168-9525(02)02837-8.PubMedView ArticleGoogle Scholar
- Lynch M, Force A: The probability of duplicate gene preservation by subfunctionalization. Genetics. 2000, 154: 459-473.PubMedPubMed CentralGoogle Scholar