Assembly and characterization of heterochromatin and euchromatin on human artificial chromosomes

Table 1 Artificial chromosome segregation errors

		Number (%) of chromosome mis-segregation events*
Line	Number of cells analyzed	Artificial	NDJ	Lag	Host 17	Host X	NDJ	Lag
17-B12	86	5/208 (2.4)	3	2	3/295 (1.0)		2	1
17-C20	224	91/745 (12.2)^†	31	60	13/884 (1.5)		9	4
X-4^‡	400	16/866 (1.8)	10	6		25/1,596 (1.6)	20	5
X-5^‡	400	43/1,954 (2.2)	30	13		4/1,588 (0.2)	4	0

*Chromosome segregation errors (either artificial chromosomes or host chromosomes 17 or X) were nondisjunction (NDJ) or anaphase lag (Lag) events. ^†The predominant artificial chromosome segregation error in 17-C20 was due to anaphase lag (66%, n = 91). ^‡Data for X-4 and X-5 have been published [14]. Segregation errors that could not be classified (for example, 1:0) were excluded from these analyses.

ISSN: 1474-760X