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Figure 4 | Genome Biology

Figure 4

From: CpG content affects gene silencing in mice: evidence from novel transgenes

Figure 4

Correlation between an absence of methylation within the EFIα promoter and expression. Methylation of EF1α sequences of the genomic DNA extracted from (a) liver and from (b) testis of animals hemizygous for the transgene. DNA was digested with XhoI, which recognizes one site in the transgene and one site in the integration locus (X) (except for the YacEF1αLacZ, for which the two XhoI are in the transgene) and with HpaII or MspI as indicated at the top. The XhoI-MspI digestion liberates a 626-bp-long fragment indicative of sites within the first intron and exon of EF1α and two 166 and 167-bp-long fragments indicative of sites within the promoter region. The reference of the transgenic line is indicated at the top. (c) All four EF1α reporter genes constructs share the same promoter region and the exon 1 (e1), the intron 1 and a part of exon 2 (e2) of EF1α. Each vertical bar corresponds to an HpaII (MspI) recognition site. Pp, EF1α probe.

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