Figure 2
From: CpG content affects gene silencing in mice: evidence from novel transgenes
Reduced CpG content in LacZ abolishes gene silencing in somatic tissues. (a-h) YacEF1αLacZ, EF1αLacZ, EF1αLagZ and EF1αLagoZ transgene expression in E9.5 representative postimplantation embryos: (a-d) in toto X-gal stained E9.5 embryos, (e-h) 20 μm cryostat sections at the level of the anterior CNS. (a,e) YacEF1αLacZ1, (b,f) EF1αLacZ2, (c,g) EF1αLagZ2 and (d,h) EF1αLagoZ1 transgenic lines. (i-o) Transgene expression in testis, in toto X-gal staining of the seminiferous tubules: (i-l) general view, (m-o) sectors with type A spermatogonia. (i) YacEF1αLacZ1, (j,m) EF1αLacZ2, (k,n) EF1αLagZ2 and (l,o) EF1αLagoZ1 transgenic lines. (m-o) β-gal+ type A spermatogonia (SpA). (p-r) Expression patterns at the blastocyst stage: (p) YacEF1αLacZ1, (q) EF1αLacZ2 and (r) EF1αLagZ2 blastocysts X-gal stained. Trph, trophectoderm; ICM, inner cell mass.