Figure 3

Alignment of cytochrome b561 protein sequences from plant and animal species. Conserved features are marked: TMH, transmembrane helices; conserved histidine residues, gray shading; conserved aromatic residues, yellow shading; and predicted MDHA-binding site (YSLHSW) and ascorbate-binding site (ALLVYRVFR) in boxes. Other conserved residues are marked in green. Red, small hydrophobic amino acids; green, hydroxyl or amino basic side chains; blue, acidic; purple, positively charged. Conservative changes at a specific position are marked with : under the alignment, while * indicates the perfect conservation at a position in all aligned proteins. The sequence from Hosb561-3 is unpublished (H.A.). Transmembrane helices were predicted with TMHMM software [43].