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Table 1 Cell strains

From: Differential gene-expression patterns in genital fibroblasts of normal males and 46,XY females with androgen insensitivity syndrome: evidence for early programming involving the androgen receptor

Cell strain Subtype, origin External genitalia/ phenotype Age at biopsy (years; months) Androgen receptor
N-33 GSF, foreskin Normal fertile male 51;11 Normal (Kd 0.08 nM, Bmax 26.40 fmol/mg protein)
N-40 GSF, foreskin Normal male 7;11 Normal (Kd 0.09 nM, Bmax 32.44 fmol/mg protein)
N-52 GSF, foreskin Normal male 0;3 Normal (Kd 0.06 nM, Bmax 35.36 fmol/mg protein)
N-56 GSF, foreskin Normal fertile male 42;10 Normal (Kd 0.07 nM, Bmax 13.35 fmol/mg protein)
N-57 GSF, foreskin Normal male 5;11 Normal (Kd 0.05 nM, Bmax 24.13 fmol/mg protein)
N-58 GSF, foreskin Normal male 5;11 Normal (Kd 0.10 nM, Bmax 30.61 fmol/mg protein)
N-59 GSF, foreskin Normal male 0;8 Normal (Kd 0.07 nM, Bmax 40.01 fmol/mg protein)
N-60 GSF, foreskin Normal male 5;4 Normal (Kd 0.05 nM, Bmax 20.08 fmol/mg protein)
N-62 GSF, foreskin Normal male 2;0 Normal (Kd 0.08 nM, Bmax 86.16 fmol/mg protein)
N-AKPZF PRF, peripheral zone Normal male Adult Not investigated
N-ST4 ASF, abdominal skin Normal male 46;0 Not investigated
N-LS12 FSF, forearm skin Normal male 36;0 Not investigated
ARD1097 GSF, labia majora Normal female 1;3 CAIS, Pro390Ser + Arg855Gly, negative androgen binding
ARD411 GSF, labia majora Normal female 0;4 CAIS, Arg855Cys, negative androgen binding
ARD682 GSF, labia majora Normal female 14;10 CAIS, no mutation in AR-gene, negative androgen binding, no AR-protein in western immunoblot
ARD402 GSF, labia majora Normal female 1;0 CAIS, no mutation in AR-gene, negative androgen binding, very low AR-mRNA transcription, no AR-protein in western immunoblot
ARD377 GSF, labia majora Predominantly female 1;2 AIS4, Ile841Ser, (Kd 0.55 nM; Bmax 17.01 fmol/mg protein)
ARD842 GOF, gonad Normal female 38;0 CAIS, 26 bp deletion exon 1 (141-150), frameshift, premature stop codon, negative androgen binding
ARD1004 GOF, gonad Normal female 17;4 CAIS, Val866Met, negative androgen binding
ARD291 GOF, gonad Normal female 18;4 CAIS, Phe794Ser, negative amdrogen binding
ARD531 GOF, gonad Normal female 35,2 CAIS, Ala765Thr, negative androgen binding
ARD557 GOF, gonad Normal female 6;6 CAIS, donor splice site exon 2/intron 2, negative androgen binding
ARD465 GSF, labia majora Normal female 5;5 CAIS, Glu287stop, low expression of wild-type AR (Kd 0.11 nM; Bmax 3.6 fmol/mg protein), post-zygotic mutation (somatic mosaicism)
ARD364 GSF, labia majora Predominantly female 23;0 AIS4, Leu 172 stop, high expression of wild-type AR (Kd 0.06 nM, Bmax 22.6 fmol/mg protein), post-zygotic mutation (somatic mosaicism) (6)
  1. ARD, strain-ID; ASF, abdominal skin fibroblast; FSF, forearm skin fibroblast; GOF, gonadal fibroblast; GSF, genital skin fibroblast; PRF, prostate fibroblast. Normal ranges for androgen (methyltrienolone) binding: Bmax (binding capacity): 13.35-115.98 fmol/mg protein; Kd (dissociation constant): 0.03-0.13 nM.