Figure 3

Hierarchical cluster analysis of genes and experiments with different DHT-treatment regimens. Shown are 686 transcripts whose log₂ red/green ratio differed from the mean expression level across all experiments by at least 1.5 in at least three experiments. The analysis is based on 2,862 transcripts identified by SAM analysis that distinguish between normal genital skin fibroblasts and gonadal fibroblasts from 46,XY female AIS patients, and between proliferating and confluent fibroblasts. The color code in the dendrogram depicts the origin of the fibroblast cultures. The gray and white bars at the top of the cluster indicate the proliferation state of the samples. On the right, the regions of the cluster diagram that differentiate between normal and AIS-derived fibroblasts, and proliferating and confluent cells, respectively, are indicated. No differences in transcript levels could be discerned between DHT-treated and control cells in either normal foreskin fibroblasts or fibroblasts from AIS-affected 46,XY females. Abbreviations are as in Figure 2 and Table 1. The scale ranges from -8 to +8 in log₂ space. For the dataset of 686 genes used for the cluster analysis and the complete dataset of 2,862 genes, see Additional data files and [36].