Figure 1

Electron micrograph of detergent-resistant membranes from Jurkat T cells prepared using 1% Triton X-100 on ice for 15 min (TX-DRMs). Vesicles of varying sizes can be seen. DNA was sheared, the lysate was mixed with 1 ml 80% sucrose and overlaid with 30% and 5% sucrose followed by centrifugation at 200,000 × g for 16 h. The DRM fraction was collected from the 5%-30% sucrose interface and samples were adsorbed to carbon films and negatively stained with 1% sodium silicotungstate. The scale bar represents 200 nm.