Table 1 Optimal probe lengths, probe positions, and formamide concentrations for purposes of exon monitoring, exon edge detection, and exon-exon junction monitoring
| Â | Exon monitoring | Exon-edge detection | Junction monitoring |
|---|---|---|---|
Probe size | 50 to 60 nucleotides | 35 nucleotides | 35 to 40 nucleotides |
Probe position | Exon interior | = 5-nucleotide steps | Centered ± 5 nucleotides |
Formamide concentration | 35% | 30% | 30 to 40% |