Identification of candidate downstream genes for the homeodomain transcription factor Labial in Drosophila through oligonucleotide-array transcript imaging

Table 1 Genes differentially expressed in response to lab overexpression

Functional class	Genes on the array (N)	Differentially expressed transcripts (n)	n/N × 100 (%)	Down-regulated	Up-regulated
Signal transduction	107	5	4.7	2	3
Transcriptional regulation	263	20	7.6	14	6
Cell cycle	37	5	13.5	0	5
Cytoskeleton/structural proteins	149	5	3.4	4	1
Metabolism	315	13	4.1	6	7
Translation	59	1	1.7	1	0
Heat-shock proteins	18	^*	^*	^*	^*
Transcription/replication/repair	73	4	5.5	0	4
Proteolytic systems/apoptosis	62	12	19.4	1	11
Cell surface receptors/CAMs/ion channels	181	12	6.6	10	2
Transposable elements	35	4	11.4	3	1
Chromatin structure	36	4	11.1	2	2
RNA binding	59	7	11.9	2	5
Secreted proteins	34	2	5.9	2	0
Unknown function	85	2	2.4	1	1
	ΣN = 1513	Σn = 96		48	48

Genes that are differentially expressed following heat-induced ubiquitous overexpression of lab in stage 10-17 hs-lab embryos, grouped according to functional classses. ^*The functional class 'heat-shock proteins' was excluded from the analysis (see Materials and methods). N, number of genes within a functional group present on the chip; n, number of genes differentially expressed within a functional group following lab overexpression; n/N × 100, number of differentially expressed genes within a functional class following lab overexpression, given as a percentage of the total number of genes in this class present on the array; downregulated, total number of genes within each functional class differentially downregulated following lab overexpression; upregulated, total number of genes within each functional class differentially upregulated following lab overexpression.

ISSN: 1474-760X