Figure 7From: Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutionsInvestigation of partial concentration and absolute concentration detection limits. Six antibody mixes were added to FCS solutions to produce a 10-fold and 100-fold increase in total protein concentration and a corresponding decrease in partial concentration. The high protein solution was diluted 10-fold, so that the total protein concentration was equivalent between the mixes. The images at the top of the figure present a detail from one of the arrays in each set. The median log10 Cy5/Cy3 fluorescence ratio is plotted as a function of cognate antibody for the three indicated antigens. The dashed line represents the log10 of the true ratio of antigen concentrations in the Cy5-labeled and Cy3-labeled solutions.Back to article page