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Figure 7 | Genome Biology

Figure 7

From: Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions

Figure 7

Investigation of partial concentration and absolute concentration detection limits. Six antibody mixes were added to FCS solutions to produce a 10-fold and 100-fold increase in total protein concentration and a corresponding decrease in partial concentration. The high protein solution was diluted 10-fold, so that the total protein concentration was equivalent between the mixes. The images at the top of the figure present a detail from one of the arrays in each set. The median log10 Cy5/Cy3 fluorescence ratio is plotted as a function of cognate antibody for the three indicated antigens. The dashed line represents the log10 of the true ratio of antigen concentrations in the Cy5-labeled and Cy3-labeled solutions.

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