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Table 1 Summary of the different protocols

From: Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analyses

Method

Efficiency (%)

Total cell number required for 5 × 106 doubly transformed cells or zygotes

Successive double transformation

0.11

4.5 × 109

Mating of yeast strains

2.8

1.8 × 108

Mating using low pH media and high cell density

10

5.0 × 107

Enhanced mating using optimized bait:prey ratio

17

2.9 × 107

  1. The efficiency of obtaining doubly transformed cells or zygotes, and the total cell number needed to gain 5 × 106 doubly transformed cells or zygotes are shown.