From: Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analyses
Method | Efficiency (%) | Total cell number required for 5 × 106 doubly transformed cells or zygotes |
---|---|---|
Successive double transformation | 0.11 | 4.5 × 109 |
Mating of yeast strains | 2.8 | 1.8 × 108 |
Mating using low pH media and high cell density | 10 | 5.0 × 107 |
Enhanced mating using optimized bait:prey ratio | 17 | 2.9 × 107 |