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Figure 3 | Genome Biology

Figure 3

From: Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analyses

Figure 3

Mating efficiency and mating competence is dependent on the ratio of the two yeast strains. Melted aliquots of YTS1/pCL1 were combined with varying numbers of freshly raised PJ69-4A/pTS195.1 cells and treated according to the protocol with preincubation at low pH and high cell density. Consistency of the final cell suspension was determined by propagating aliquots onto selective medium and counting cell numbers in a hemocytometer. Filled diamonds, mating competence, estimated as the percentage of zygotes per viable MATα cells. crosses, mating efficiency, estimated as the percentage of zygotes per total cell number.

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