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Figure 1 | Genome Biology

Figure 1

From: Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analyses

Figure 1

Successive transformation of yeast strain PJ69-4A. Yeast strain PJ69-4A was transformed with vector pTS195.1, raised in liquid selective medium lacking uracil (SD-U) and then successively transformed with plasmid pCL1. Numbers of doubly transformed cells were monitored by propagating aliquots on selective medium lacking leucine and uracil (SD-LU) and efficiency in terms of doubly transformed cells versus all cells was calculated.

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