Figure 1From: Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analysesSuccessive transformation of yeast strain PJ69-4A. Yeast strain PJ69-4A was transformed with vector pTS195.1, raised in liquid selective medium lacking uracil (SD-U) and then successively transformed with plasmid pCL1. Numbers of doubly transformed cells were monitored by propagating aliquots on selective medium lacking leucine and uracil (SD-LU) and efficiency in terms of doubly transformed cells versus all cells was calculated.Back to article page