Skip to main content
Figure 5 | Genome Biology

Figure 5

From: Sources of nonlinearity in cDNA microarray expression measurements

Figure 5

Comparison of software packages for image analysis. Each panel shows theoretical intensity levels (as fractions of the initial concentration in the dilution series) or measured intensity values (in arbitrary units) for the radioactively labeled dilution experiment. ImageQuant was used on the original GEL file; GLEAMS and ArrayVision were used on the TIFF file produced from the GEL file by rotation and cropping. (a) ImageQuant values are linearly related to the theoretical values. (b) The values reported by GLEAMS and ArrayVision are linearly related. (c) The values reported by ArrayVision are not linearly related to the theoretical values. In this case, both ArrayVision and GLEAMS provide measurements related to the square root of the theoretical values.

Back to article page