| Â | Non-Ind | Ind (1) | Ind (2) | Ind (3) | Ind (4) |
|---|
Test gene |
n
| % Phe |
n
| % Phe |
n
| % Phe |
n
| % Phe |
n
| % Phe |
|---|
gpb-1
| 546 | 0 | 530 | 100 | 309 | 84 | 442 | 97 | 346 | 0 |
unc-22
| 422 | 0 | 255 | 99 | 179 | 80 | ND | ND | ND | ND |
par-1
| ND | ND | 313 | 100 | 263 | 100 | ND | ND | ND | ND |
par-3
| ND | ND | 391 | 96 | 325 | 11 | ND | ND | ND | ND |
- Four different methods were compared to determine optimal induction conditions for RNAi; non-induced (Non-Ind) bacteria were also included for comparison. Induction conditions (Ind) were as follows: (1) Bacteria were induced on plates with IPTG at room temperature overnight; (2) bacteria were induced in culture at 37°C for 2 h; (3) bacteria were induced on plates with IPTG at 37°C overnight; (4) bacteria were induced in culture at 37°C overnight (see the Materials and methods section for detailed protocols). gpb-1, par-1 and par-3 were scored for percentage of dead embryos, unc-22 was scored for percentage of worms with an uncoordinated phenotype. Data shown represent the progeny of three fed worms. ND, not done; n is the number of worms or embryos scored; %Phe, percentage of worms or embryos with phenotype.
