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  • Web report
  • Open Access

A database of restriction endonuclease buffers

Genome Biology20001:reports232

https://doi.org/10.1186/gb-2000-1-1-reports232

  • Received: 10 January 2000
  • Published:

Abstract

This simple but incredibly useful tool for molecular biologists allows the user to select one or more restriction endonuclease enzymes and determine which of the enzyme buffers provided by New England Biolabs, Boehringer Mannheim, Gibco BRL, Fermentas or Stratagene can be used in a restriction enzyme digest.

Keywords

  • Restriction Enzyme
  • Search Method
  • Restriction Digest
  • High User
  • Commercial Enzyme

Content

This simple but incredibly useful tool for molecular biologists allows the user to select one or more restriction endonuclease enzymes and determine which of the enzyme buffers provided by New England Biolabs, Boehringer Mannheim, Gibco BRL, Fermentas or Stratagene can be used in a restriction enzyme digest.

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Reporter's comments

Timeliness

Last updated 1998.

Best feature

The Multi-Cut database can eliminate the frustration and wasted time spent looking through multiple vendor catalogs or websites looking for buffer information, and eliminates the guesswork in determining which buffers to use for double restriction digests.

Wish list

The only thing that you could wish for is to have all commercially available restriction enzymes and their buffers listed in the database, and the activity of all combinations of enzymes and buffers experimentally confirmed, rather than predicted.

Related websites

New England Biolabs hosts REBASE, the restriction enzyme database, which contains information about all known restriction enzymes. Buffer information for commercial enzymes is available, but there is no search method to find compatible buffers for different enzymes.

Table of links

References

Copyright

© BioMed Central Ltd 2000

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