Figure 1

Phenotype phase diagram of the H. influenzae Rd metabolic phenotype (reprinted with permission from the Journal of Biological Chemistry).

The figure illustrates the six distinct metabolic phenotypes derived from the H. influenzae Rd in silico metabolic genotype when optimized for maximal biomass production under different conditions (mixed substrate with varied glutamate and fructose uptake rates). These metabolic phenotypes employ different combinations of pathways to optimize growth depending upon substrate availability, each with different constraining features that reflect the critical components (such as ATP) defined by connectivity analysis. In addition, they illustrate the complex relationship between pathway utilization and growth conditions.